White Paper: Transfection of siRNAs into CML Primary Cells Using Nucleofection®
نویسنده
چکیده
RNA interference studies are based on transfection of short oligonucleotides (siRNAs) into cells and transfection effi ciency is a crucial factor for such experiments. Most conventional transfection methods are suitable for immortalized cell lines, but are not effi cient in primary cells. We performed comparative analysis of transfection methods for siRNA delivery into peripheral blood leukocytes from chronic myeloid leukemia (CML) patients. In conclusion, transfection by Amaxa® Nucleofector® Technology represented the best and only suitable non-viral method for transfection of CML primary cells. Using Nucleofection®, we successfully transfected ~40% of the cells and achieved signifi cant knockdown (~78%) of the target genes. Moreover, the cell viability was not aff ected as with other methods.
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